De novo biosynthesis of tyrosol acetate and hydroxytyrosol acetate from glucose in engineered Escherichia coli

• Tyrosol acetate and hydroxytyrosol have wide applications both as functional food components nutraceuticals. Construction of tyrosol biosynthetic pathway in E. coli . derived from virgin olive oil olives extract, However, they low bioavailability due to their absorption high metabolism human liver small intestine. Acetylation can effectively improve thus increase potential use the cosmeceutical industries. There is no report on bioproductin so far. Thus, it great significance develop microbial cell factories for achieving or biosynthesis. In this study, a de novo production was constructed Escherichia First, an engineered that allows simple carbon sources established. Four aldehyde reductases were compared, found yeaE best reductase accumulation. Subsequently, extended by further overexpression alcohol acetyltransferase ATF1 conversion acetate. Finally, 4-hydroxyphenylacetate 3-hydroxylase HpaBC.